These consumables (ThermoFisher), are optimized for this machine, and have been validated by our lab:
Sybr Green: https://www.thermofisher.com/order/catalog/product/A46113
Plates: https://www.thermofisher.com/order/catalog/product/4483354?SID=srch-srp-4483354
Film: https://www.thermofisher.com/order/catalog/product/4311971?SID=srch-srp-4311971 (VERY IMPORTANT. Other film brands like BioRad might melt with use and leave residue in the machine)
Plate support (need to set up the reaction plate and prevent contamination of the machine due to particulates): https://www.thermofisher.com/order/catalog/product/4312063?SID=srch-hj-4312063

Manual to the QuantStudio 5 qPCR machine
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017162_QS5HIDInstrument_UG.pdf